Identification of genes regulated by dark adaptation and far-red light illumination in roots of Arabidopsis thaliana.
Sato-Nara K, Nagasawa A, Yamashita H, Ishida J, Enju A, Seki M, Shinozaki K and Suzuki H
Roots in the soil are illuminated by far-red (FR) light passed through plant tissues in the daytime, and are in complete darkness at night. To evaluate whether gene expression of roots is affected by a dark-FR light cycle, gene expression profiles were analysed for dark-adapted versus light-grown plants and for FR light-illuminated versus dark-adapted plants using the RIKEN Arabidopsis full-length cDNA microarray (containing approximately 7000 independent, full-length cDNA groups). Among candidate dark- and FR-regulated genes, several were further analysed. Eleven dark-inducible and five dark-repressed genes were characterized. Almost all the dark-inducible and -repressed genes were oppositely regulated by FR light illumination. The functions of dark- and FR-responsive genes and the significance of FR light-regulated gene expression in roots under ground are discussed.
Plant Cell Environ. (2004) 27(11):1387-94